FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19460931
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC29750.080.379961
TTACGCG10950.080.106989
ACGACCG31500.079.6288761
CGCCGAA30900.079.281781
TGCGCGA29750.078.167671
CGGTACG31850.076.45781
GGCGACT39600.071.4665451
GCGCTAG33000.070.9125441
GACGCGT29750.070.793361
CCGCTAT35200.067.727021
GTGCGAC32750.067.211291
AACCGCG36800.066.968271
GCGCGTA33450.066.897871
GTACCGT46600.065.4932258
ATAACGC34900.065.166141
CGAACGC39150.064.816371
TCACGCG25400.062.50448
CGTTGCG15100.061.9316987
CCGATAA37500.061.427991
GCCGGAT39450.061.1721761
Produced by FastQC (version 0.11.5)