FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20190454
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13650.079.6623159
CGCCGAA30350.076.400841
TTTGCGC30800.074.8096161
GTGCGAC35000.072.102221
ACGACCG32600.072.025291
CGGTACG30950.071.3746261
GCGCTAG33300.070.730911
TGCGCGA30150.070.114551
TTACCCG39350.069.5223541
TAGGCGC36300.069.1170351
CCGCTAT40400.068.620751
GACGCGT33200.068.079751
ATAACGC38200.066.828181
CCTTACG37250.065.390631
TCCGCGA30300.065.315538
TTCCGCG19100.064.902177
GTACCGT44150.064.8578958
TCGTACG40100.064.357938
CGAACGC37650.063.9187851
GGCGACT42700.063.896721
Produced by FastQC (version 0.11.5)