FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17614048
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG26650.078.531221
TTACGCG10000.078.291989
TGCGCGA25200.076.371041
GTGCGAC32800.076.3002241
CGGTACG27850.075.410221
CGCCGAA25700.074.031021
TTTGCGC29150.073.302341
ACGCCGA36550.069.472921
GCGCGTA27500.067.322671
GCCGGAT33450.065.84821
CGAACGT18500.065.4405443
GACGCGT25000.065.273721
GCGCTAG29450.065.101281
CGAACGC34850.064.8827441
GTACCGT36900.063.6520168
TTCCGCG16100.063.487287
TCGCGAT19450.062.6156928
GGCGACT37100.062.5257451
TTACCCG33950.062.2919041
ATAACGC34750.062.1213261
Produced by FastQC (version 0.11.5)