FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18582174
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1775153595.52991485280464No Hit
NAGTAGAG2925031.5741053764753252No Hit
AAGGAGAG699840.3766190113169751No Hit
AAGTCGAG659460.35488850766331215No Hit
AAGTAGCG397690.2140169390298466No Hit
AAGTTGAG382030.2055895074494513No Hit
AAGTGGAG374770.2016825372531761No Hit
AAGCAGAG365250.19655934768450667No Hit
ACGTAGAG352920.18992395615281613RNA PCR Primer, Index 17 (100% over 8bp)
AAGAAGAG332960.1791824788638832No Hit
AGGTAGAG234700.1263038436729739No Hit
AAGTAGAA206540.11114953503287614No Hit
AAGTAGGG204340.1099656046703685No Hit
CAGTAGAG200840.1080820790936518No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)