FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18329556
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12500.092.7916269
TTTGCGC31750.076.2533341
GTGCGAC31800.075.21341
TGCGCGA27900.073.4054641
CGCCGAA30400.071.6996841
ACGACCG25550.070.996251
CGGTACG29100.068.116271
TCGTACG35500.067.796888
TTACCCG36500.067.532311
GCGCTAG30100.067.311281
GTTACGC31250.066.346198
CGAACGC34000.065.398721
GCGCGTA32000.065.143261
GTTCGCG37100.064.286838
TACGAAC39950.063.8974041
CCTTACG35200.062.1302531
GACGCGT28450.061.702611
GCCGGAT38650.061.693961
GCGCAAC41950.061.1997571
TAGGCGC35550.061.1070671
Produced by FastQC (version 0.11.5)