FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18871123
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26200.094.906056
ACGACGC27100.092.289195
CACGACG30100.084.05614
GACGCTC40450.062.009567
TACACGA44400.057.487672
CTACACG46050.056.0826951
ACGCTCT49750.050.272118
ACACGAC52300.048.9305233
CGCTCTT63750.039.3456769
TCTTCCG91850.06.59809110-14
CTTCCGA96950.06.49027510-14
TCGCCGG24905.041329E-86.4048743145
TAACCCT706050.05.94489536
CGTTGAG25051.8746687E-65.8745371
ACCGGAG25302.2362328E-65.81648871
TTCCGAT121650.05.792223510-14
CTAACCC749950.05.7902275
CGCTAAG16600.00204690275.76217751
AACCCTA761950.05.6684331
ACCCTAA774600.05.64488172
Produced by FastQC (version 0.11.5)