FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19025194
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30100.079.935321
TTTGCGC32250.077.554271
CGGTACG31150.073.014921
TTACGCG12500.072.49489
CGCCGAA32050.072.333681
GTGCGAC32600.072.2349851
ACGACCG26900.072.0448461
GCGCTAG33000.071.8026351
GACGCGT29050.070.992471
TTACCCG37250.066.947971
ATAACGC36900.065.997461
TAGGCGC34350.065.787151
GCGCGTA31550.065.598881
CGAACGC34900.064.540831
CGAACTA38900.063.5442731
ACGCCGA38600.063.4697531
CCGCTAT38100.063.342951
TTCCGCG17650.062.02127
GTTACGC28550.061.9570168
GTACCGT41450.061.9135258
Produced by FastQC (version 0.11.5)