FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19025194
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT60200.0118.375376
ACGACGC60700.0117.9980555
CACGACG61700.0116.20254
GACGCTC72650.098.091247
TACACGA75200.095.1037142
CTACACG75950.095.068621
ACGCTCT79700.089.501858
ACACGAC83150.086.661043
CGCTCTT96450.074.634959
TCTTCCG114200.013.40682710-14
CTTCCGA119050.013.22600310-14
TTCCGAT133450.011.92921710-14
TCCGATC135250.011.24517415-19
CGATCTA39100.011.05013215-19
CCGATCT143850.010.855115-19
GCTCTTC224300.07.129751710-14
CGATGGG36004.1836756E-116.12734371
CGATCTG71850.06.073900715-19
CGTAGAG27602.8660725E-75.86093761
CGATCTT69300.05.858045615-19
Produced by FastQC (version 0.11.5)