FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18773133
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28100.096.99966
ACGACGC28000.096.828245
CACGACG30100.090.072074
GACGCTC40300.067.6349647
TACACGA44550.061.722642
ACGCTCT47400.057.656778
CTACACG48650.057.469331
ACACGAC51650.052.7717363
CGCTCTT61600.044.483449
TCTTCCG83450.07.470850510-14
CTTCCGA89700.07.273576710-14
CGCTGAG36850.07.1878731
TAACCCT710400.06.65323836
TTCCGAT107450.06.40936710-14
CTAACCC757750.06.3714285
CCCTAAC785450.06.25741533
ACCCTAA780350.06.21999032
CCTAACC782850.06.1856154
AACCCTA772000.06.15673731
TCCGATC109450.06.02717315-19
Produced by FastQC (version 0.11.5)