FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21605407
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCAGA2084021596.45833100945518No Hit
NGTCCAGA3481721.611504009158448No Hit
GGTCCAGC512770.23733410807766775No Hit
GGTCCCGA353550.16363959262604957No Hit
GGCCCAGA309390.14320026463745858No Hit
GGGCCAGA289370.13393406567161636No Hit
GGTCCAGG238150.11022703714861748No Hit
GGTCCAGT236330.10938465542445No Hit
GGTTCAGA226070.10463584416623116Illumina DpnII expression Adapter 1 (100% over 8bp)
TGTCCAGA224560.10393694504343287No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)