FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17878607
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG29200.076.6174551
TGCGCGA27050.076.490651
GTGCGAC32300.075.601971
TTACGCG10950.075.474039
TTTGCGC28200.074.926921
CGCCGAA24800.072.817281
CGGTACG26200.071.9958651
ATAACGC32250.068.918061
GCGCTAG29600.068.666081
CCGCTAT35450.068.265441
TCGTACG34550.066.934418
GACGCGT28850.066.903261
TAGGCGC31400.066.1268541
GCGCGTA29300.065.1271441
AACGCGT24450.064.34353
CGAACGC33750.064.122011
GGCGACT37000.063.8248751
GCCGGAT34550.063.0604971
TTACCCG36250.062.5234341
TCGCGAT21950.062.4214868
Produced by FastQC (version 0.11.5)