FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17878607
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23950.098.073276
ACGACGC24400.095.967435
CACGACG26600.087.758484
GACGCTC36750.064.7029347
TACACGA41200.058.4365352
CTACACG42400.056.5623631
ACGCTCT45700.052.0313578
ACACGAC47450.049.9604533
CGCTCTT55200.043.0769279
TCTTCCG75750.07.10110810-14
CTTCCGA80500.07.078341510-14
CGATGAG28001.3915269E-96.54828551
TTCCGAT95900.06.28940510-14
TCCGATC94850.06.114410415-19
TAACCCT682100.05.9943526
CTAACCC722950.05.82611475
AACGGAG35351.1423253E-95.8091721
AACCCTA731950.05.73139331
CCCTAAC752600.05.7122253
CCGATCT103550.05.670702515-19
Produced by FastQC (version 0.11.5)