FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18508478
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11900.079.195439
TGCGCGA25850.078.349171
GTGCGAC32100.075.394241
CGCCGAA29650.073.7329561
GCGCTAG32100.073.572021
TTTGCGC30600.073.355411
CGGTACG28100.072.335861
GACGCGT29450.072.2475051
ACGACCG22950.069.134091
AACGCGT26150.067.920983
TCGCGAT21150.065.467648
TTACCCG34450.065.157491
GTACCGT41950.064.976988
CCGCTAT36600.064.726051
ATAACGC35950.064.472661
CGAACGC32700.064.396161
GGCGACT35100.064.159171
TCGTACG34150.064.109138
GTTACGC28300.063.0162248
TAGGCGC37500.062.5876961
Produced by FastQC (version 0.11.5)