FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17925666
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11650.085.250689
CGCCGAA26100.079.0220261
TGCGCGA25300.078.340851
GTGCGAC29500.074.624951
TTTGCGC31400.074.069091
CGGTACG29500.072.889491
ACGACCG26600.072.58751
GCGCTAG29400.069.405911
GACGCGT25250.066.330551
CGAACGC33500.064.186261
CCGCTAT35650.063.80291
ACGCCGA33550.063.6546131
GTCGGAC28500.063.6423761
TCGTACG36150.063.3706258
GCCGGAT36400.063.2919851
CGAACTA39700.063.1892471
CCGAACG18550.062.5286759
GTTACGC29500.062.173858
GTTCGCG38600.062.1655658
ATAACGC33200.061.682151
Produced by FastQC (version 0.11.5)