FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18713637
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC28100.0100.096445
CGACGCT28750.097.582286
CACGACG29450.095.7549064
GACGCTC40500.069.450387
TACACGA43300.066.19712
CTACACG45150.063.8485341
ACGCTCT48450.058.2041178
ACACGAC52150.055.04753
CGCTCTT62450.045.27249
TCTTCCG83700.07.50047810-14
CTTCCGA91650.07.27699110-14
TCGCCGG23651.8169885E-86.743419145
CGTAGAG30201.546141E-106.57475331
TTCCGAT105500.06.541554510-14
CGATGAG29454.0290615E-96.242771
TCCGATC107100.06.22721315-19
TAACCCT751300.06.1367956
CGTAAAG25704.696485E-76.00909141
CTAACCC796900.05.92202765
CCGATCT118300.05.919536615-19
Produced by FastQC (version 0.11.5)