FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16495719
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26700.083.325261
CGGTACG27700.078.2035141
TTACGCG11800.075.564899
ACGACCG26600.074.834461
TTTGCGC27250.074.6608051
CGCCGAA26600.074.009081
TTACCCG32500.070.0311661
GACGCGT23750.069.640071
GCGCGTA23300.069.414581
GTGCGAC28400.068.029921
GGCGACT34050.067.917911
GCGCTAG28300.066.718711
ATAACGC31100.064.2416461
CGATTCG19250.064.020884
GCCGGAT33500.063.5715141
CGAACGT15450.062.875263
AACGCGT22500.062.183943
CCGCTAT29800.062.1324651
TCGTACG32550.061.9140748
GTTACGC26350.061.9009748
Produced by FastQC (version 0.11.5)