FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15909201
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24450.099.9198766
ACGACGC25000.098.011625
CACGACG26700.091.771764
GACGCTC35400.069.6265957
TACACGA37400.066.3132
CTACACG39250.062.7907681
ACACGAC42550.058.438013
ACGCTCT45100.054.4907348
CGCTCTT55350.044.006949
TCTTCCG73950.07.62671310-14
CTTCCGA77400.07.56774310-14
CGTAGAG22907.1140676E-97.06769561
TTCCGAT91850.06.882294710-14
TCGCCGG16153.5708938E-56.7329583145
CGATCTA26700.06.516227215-19
TCCGATC93650.06.362975615-19
CGATGAG25455.7987563E-86.35953761
AACGGGG27958.589268E-96.31713341
CCGATCT100350.06.24155215-19
CGAAAAG29955.798938E-96.14092541
Produced by FastQC (version 0.11.5)