FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15757083
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21750.0101.6610646
ACGACGC22350.099.904695
CACGACG23750.093.7109154
GACGCTC32450.068.809337
CTACACG36150.062.3553851
TACACGA37850.059.383962
ACACGAC41150.054.2612083
ACGCTCT42800.052.1698538
CGCTCTT54000.041.080949
CGTTGAG22104.5110937E-107.64151531
TCTTCCG73400.07.32846710-14
CTTCCGA78850.07.26324410-14
TTCCGAT93300.06.29373810-14
CGCAAAG23506.213486E-76.24893571
AACGGGG27253.527748E-86.19733951
CGATCTA25750.06.137295715-19
TAACCCT502250.06.1201216
TCCGATC91750.06.068103315-19
CTAACCC528800.06.03217555
CCTAACC550450.05.9134914
Produced by FastQC (version 0.11.5)