FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18156804
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12150.082.936469
TTTGCGC27700.078.932671
TGCGCGA26450.075.751331
ACGACCG26650.071.890161
TTACCCG34700.071.860511
GACGCGT28350.070.674521
CGCCGAA29250.070.2499161
CGGTACG26150.069.349821
GTGCGAC33050.068.589181
GTTACGC27200.067.697378
GTTCGCG35650.065.4791958
TCGTACG35350.065.009518
GCGCTAG31700.064.820511
ATAACGC34750.064.8128741
CGAACGC34400.063.9843031
CGAACTA40600.063.0387151
CCGATAA38750.062.462831
GGCGACT34500.062.3151471
GCGCGTA28600.062.130611
CGCGGTA34750.062.0772631
Produced by FastQC (version 0.11.5)