FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18156804
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23200.094.055646
ACGACGC23450.093.6714555
CACGACG26550.082.734314
GACGCTC33350.065.8654867
TACACGA37050.060.2957342
CTACACG43300.051.7870981
ACGCTCT43900.050.3644078
ACACGAC51300.043.6661643
CGCTCTT57150.039.3218429
TCTTCCG76100.06.68725310-14
CTTCCGA77900.06.68162810-14
CGATGGG35553.092282E-116.20427851
TTCCGAT91000.06.165877310-14
TAACCCT630300.06.15336666
TCCGATC91100.06.00000215-19
CGTTGAG25805.074271E-75.9842431
CGATGAG27202.1616688E-75.9465271
CTAACCC674000.05.9050015
CGATGGT45300.05.8427051
CCTAACC694500.05.7724534
Produced by FastQC (version 0.11.5)