FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17818932
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT20000.084.095346
ACGACGC21650.078.6907965
CACGACG23500.073.1129764
GACGCTC32050.053.1564837
TACACGA36300.048.7403452
CTACACG41100.044.0049131
ACGCTCT40550.042.0139438
ACACGAC44700.039.410573
CGCTCTT54450.031.6880479
CTTCCGA77550.06.169871310-14
TAACCCT613550.05.9669916
CTAACCC649950.05.9116665
CGATGGG34103.090463E-95.7976951
CCTAACC673950.05.7872014
TCTTCCG75500.05.780474710-14
AACCCTA662350.05.77070671
ACCCTAA671700.05.71065242
CCCTAAC688100.05.7103363
AACGGGG33005.3767508E-85.5471781
CGTTGAG25151.352473E-55.53173831
Produced by FastQC (version 0.11.5)