FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842266
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA24250.082.317891
CGCCGAA25800.080.7734151
ACGACCG24900.080.169031
TTACGCG11300.078.909479
CGGTACG25250.075.582711
GCGCTAG27900.071.810781
TTTGCGC25850.071.282561
GGCGACT36700.069.335641
GACGCGT26850.069.1723861
GTGCGAC30950.068.9867251
GCGCGTA26900.067.141031
CGCGGTA34850.066.511961
GCCGGAT33250.066.193931
TTACCCG33500.065.918211
CGAACGC32300.065.650611
ATAACGC31000.065.573211
TCGTACG33050.064.9271858
CGAACGT17050.064.631973
CGTTGCG13600.064.499937
AACGCGT23500.064.168773
Produced by FastQC (version 0.11.5)