FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18691190
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27850.091.369046
ACGACGC28350.090.780235
CACGACG28450.089.442824
GACGCTC37950.067.4338157
TACACGA43200.059.245232
CTACACG48150.053.3542371
ACGCTCT48250.053.3391238
ACACGAC48900.052.0365453
CGCTCTT59250.044.2931149
TCTTCCG91100.06.875453510-14
CTTCCGA95150.06.87232610-14
TAACCCT733750.06.46169956
CTAACCC784700.06.15299465
CCTAACC807350.06.0702354
CCCTAAC811100.06.06883763
ACCCTAA804250.06.0671742
CGTTGAG26751.6120066E-76.03665071
AACCCTA793150.06.01527071
CGTTGGG33451.8535502E-95.92467831
TTCCGAT116850.05.819425610-14
Produced by FastQC (version 0.11.5)