FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19575206
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10950.083.418569
TTTGCGC31150.082.6604161
CGCCGAA29550.082.185181
TGCGCGA30000.080.708571
ACGACCG29150.074.028071
GTGCGAC33350.072.1627351
CGGTACG31200.071.743041
GCGCTAG33800.070.985571
GGCGACT40100.068.042051
TTACCCG36350.067.816981
ATAACGC37950.067.27081
GACGCGT34100.067.143341
GCGCGTA35050.065.949581
CCGCTAT36750.065.68551
GTTACGC31300.065.314718
CGAACGC37000.064.2531741
AACGCGT28050.063.8366853
TCGCGAT23650.063.145488
TAGGCGC37550.063.117241
GTTCGCG39000.061.8993158
Produced by FastQC (version 0.11.5)