FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18947969
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12650.083.095819
TGCGCGA26900.076.978631
CGCCGAA27950.075.91931
GTGCGAC33800.073.6034551
TTTGCGC28900.073.1704941
ACGACCG28600.071.891291
TTACCCG37150.070.3145751
TAGGCGC32700.068.4714661
CGGTACG29450.068.077131
GACGCGT29250.067.5421
GCGCTAG29800.066.786491
GTTACGC29600.066.371488
GCGCGTA33100.065.654491
GGCGACT38400.064.405271
GCCGGAT38550.063.7750551
AACCGCG34650.062.7175641
CGAACTA39900.062.5341841
CGCGGTA37500.062.24371
TCACGCG28450.062.174388
CGAACGT18550.061.7473373
Produced by FastQC (version 0.11.5)