FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17393829
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC22700.095.487645
CGACGCT23500.091.619776
CACGACG24650.088.815874
GACGCTC34900.061.693637
TACACGA37650.057.9873352
CTACACG42950.052.5504571
ACGCTCT42200.051.0191658
ACACGAC44900.049.8897673
CGCTCTT55400.039.2555279
TCTTCCG73850.06.989189610-14
CTTCCGA80850.06.599258410-14
CGTTGAG22502.7716487E-76.53504751
CGTTAAG19801.1191067E-56.31226161
TCGCCGG19701.2870336E-56.255429145
CGATCTA26700.06.027570215-19
TTCCGAT97450.05.65365210-14
TAACCCT431100.05.5324626
TCCGATC98000.05.518401615-19
CTAACCC445500.05.4838315
CGTAGAG26856.476257E-65.4762971
Produced by FastQC (version 0.11.5)