FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19602052
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12200.082.595499
CGCCGAA29700.078.343711
TTTGCGC31000.076.2384951
CGGTACG31300.074.338921
GTGCGAC34150.072.20591
TGCGCGA29600.071.19251
GCGCTAG32450.068.773111
GCCGGAT38400.067.8347551
TTACCCG38600.066.5354841
GCGCGTA30900.066.066181
ATAACGC37300.065.911921
TAGGCGC35900.065.628861
ACGACCG28450.065.5830151
CCGCTAT41650.065.352391
GTTACGC30750.064.8319248
ATTAGCG38400.064.7861
TTCCGCG20800.064.477897
CGATCGG36300.064.09941
GACGCGT30050.064.039031
TCGTACG37700.063.2638858
Produced by FastQC (version 0.11.5)