FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18374514
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24300.090.988486
ACGACGC25450.086.5905155
CACGACG27450.080.282224
GACGCTC36300.060.5102357
TACACGA40800.055.1324232
CTACACG42650.053.3186871
ACGCTCT43150.051.2401858
ACACGAC52300.042.5527043
CGCTCTT57700.038.1940169
CGTAGAG28000.09.19921
TCTTCCG79800.06.595268710-14
CTTCCGA80900.06.54143610-14
TAACCCT642200.06.3100736
CTAACCC679450.06.30542765
CGTTAGG22201.4966499E-66.29855061
AACCCTA694700.06.0595271
CCTAACC704500.06.0298254
CCCTAAC711900.05.9977293
ACCCTAA705400.05.9662032
CGCAGAT30555.62286E-85.78149371
Produced by FastQC (version 0.11.5)