FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19234358
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT61600.0116.39026
ACGACGC62750.0114.6016545
CACGACG63200.0114.016554
GACGCTC74050.096.919457
TACACGA76000.095.383182
CTACACG77200.094.157331
ACGCTCT83400.086.141158
ACACGAC84400.085.806783
CGCTCTT96500.074.598019
CTTCCGA121600.013.41364510-14
TCTTCCG119350.013.03482410-14
TTCCGAT133700.012.18885110-14
TCCGATC133700.011.45141115-19
CCGATCT144400.010.94424515-19
CGATCTA41850.09.97754215-19
GCTCTTC230500.06.95684110-14
CGATCTG67050.06.616817515-19
CGATCTT62750.06.446396415-19
TAACCCT759200.06.1207226
CTAACCC803450.06.08126545
Produced by FastQC (version 0.11.5)