FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17598553
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG27250.080.004741
TGCGCGA26200.076.7887041
TTTGCGC28900.076.449571
GTGCGAC30050.076.445361
CGCCGAA27100.075.588321
TTACGCG10950.074.811779
CGGTACG28450.073.287261
GCGCTAG31350.069.7749561
CGAACGC32950.068.829111
GGCGACT34000.067.133861
ATAACGC32400.066.3847351
TCGTACG33400.065.3319558
AACGCGT27350.065.2051853
TTCCGCG17200.064.907957
CCGCTAT36000.064.826731
CGTTGCG13650.064.79377
TTACCCG33550.064.7634351
AACCGCG32850.064.139131
GCGCGTA28050.062.8564871
CCGAACG16000.062.526589
Produced by FastQC (version 0.11.5)