FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567730
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC23350.099.657985
CGACGCT23750.098.2876
CACGACG26450.087.978844
GACGCTC36350.064.417217
TACACGA40000.059.8653832
ACACGAC42400.056.250833
CTACACG43550.055.402581
ACGCTCT45000.051.2294468
CGCTCTT55850.041.1473969
TCTTCCG76800.07.07940410-14
CTTCCGA80700.07.042710-14
TTCCGAT90400.06.591739710-14
TCCGATC87950.06.57749415-19
ACCGGGT20753.3627675E-66.38114641
CCGATCT96800.06.245743315-19
CGTAAAG23101.9109313E-55.7319821
CGATCTA26650.05.603552315-19
ACGGGAG37705.0549716E-95.4633751
TAACCCT452950.05.44167386
CTAACCC464800.05.3963975
Produced by FastQC (version 0.11.5)