FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15922281
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC23500.098.716125
CGACGCT23850.095.443696
CACGACG24550.094.494344
GACGCTC34000.067.805097
TACACGA39500.060.210382
CTACACG41550.056.930611
ACACGAC42650.055.922193
ACGCTCT41400.055.335128
CGCTCTT54150.042.7077149
CTTCCGA74900.07.530222410-14
TCTTCCG72350.07.27458310-14
TTCCGAT93600.06.397557710-14
TCCGATC94550.06.087744715-19
TCGCCGG18051.6571493E-46.0243154145
CGATGGT34908.6583896E-105.8755291
CCGATCT104650.05.77729215-19
ACGGGAG37156.6393113E-105.7168071
TAACCCT505450.05.6653256
ATCGGGG23452.6104766E-55.62139371
CTAACCC529450.05.6139025
Produced by FastQC (version 0.11.5)