FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18622386
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA28750.081.961211
TTACGCG11450.079.142269
ACGACCG30550.077.611151
CGCCGAA30600.077.0060351
CGGTACG31800.072.489281
TTTGCGC28900.071.6603551
GTGCGAC31600.071.5585861
GCGCGTA29500.069.706721
GACGCGT27350.069.56751
GCGCTAG31550.067.264971
CGAACGC34150.067.072421
TTACCCG36200.066.710731
ATAACGC36200.065.497811
GTTCGCG42200.065.107638
ACGCCGA39350.065.0899051
CGTTGCG14500.063.9953237
TCGTACG37950.063.611788
GTACCGT40950.062.6692478
CCGCTAT38200.062.6433141
GGCGACT36700.062.4120751
Produced by FastQC (version 0.11.5)