FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18622386
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC53600.0116.992245
CGACGCT54300.0114.54956
CACGACG55650.0113.203634
GACGCTC66250.093.559847
TACACGA68400.093.287212
CTACACG72350.088.885991
ACGCTCT74050.083.900598
ACACGAC78850.080.907123
CGCTCTT87250.071.2904059
TCTTCCG112100.012.24845410-14
CTTCCGA115400.012.19973210-14
TTCCGAT126350.011.01622710-14
TCCGATC126450.010.62905315-19
CCGATCT135000.010.22437615-19
CGATCTA40250.09.61787215-19
GCTCTTC211000.06.65165610-14
CGATCTT64450.06.208973415-19
CGTTGAG27404.150388E-86.14829351
TAACCCT690900.06.03334866
TCGCCGG22953.4145214E-66.001604145
Produced by FastQC (version 0.11.5)