FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19703799
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1879727795.39925270248646No Hit
NAGTAGAG3795071.9260600455780128No Hit
AAGGAGAG659430.3346715016733575No Hit
AAGTCGAG634970.32225765193808564No Hit
AAGCAGAG357670.1815233701886626No Hit
ACGTAGAG354810.18007187344937897RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG344660.17492058257394932No Hit
AAGTTGAG341630.1733828080564565No Hit
AAGTGGAG335660.17035293549228755No Hit
AAGAAGAG320040.16242553022389236No Hit
ANGTAGAG249460.12660502677681598No Hit
AGGTAGAG228940.11619079143062716No Hit
AAGTAGAA219690.11149626526336368No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)