FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18624263
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1759123694.45332682426145No Hit
NAGTAGAG3509591.8844181914742075No Hit
AAGGAGAG880470.4727542775786618No Hit
AAGTCGAG689800.3703770721021283No Hit
AAGTAGCG476740.2559779144012303No Hit
AAGAAGAG455020.2443157079557994No Hit
AAGTTGAG447330.24018668550803862No Hit
AAGCAGAG434350.2332172822086973No Hit
AAGTGGAG418150.22451895143448092No Hit
ACGTAGAG408540.219359015709776RNA PCR Primer, Index 17 (100% over 8bp)
AGGTAGAG380930.20453426801371952No Hit
AAGTAGGG317020.17021881617543738No Hit
AAGTAGAA231770.12444519281111957No Hit
AAGTAGTG219860.11805030889007527No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)