FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19498858
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1859614595.37043143757444No Hit
NAGTAGAG3502871.7964487971551975No Hit
AAGGAGAG647890.3322707411890481No Hit
AAGTCGAG624990.3205264636523842No Hit
ACGTAGAG380150.19496013561409598RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG372440.1910060578932366No Hit
AAGCAGAG362590.18595448000082876No Hit
AAGTTGAG350880.17994900009015913No Hit
AAGTGGAG343910.17637443177441467No Hit
AAGAAGAG320470.16435321494212637No Hit
ANGTAGAG320260.1642455163271613No Hit
AGGTAGAG239070.12260718037948684No Hit
AAGTAGAA219410.11252453861656923No Hit
CAGTAGAG198630.10186750424050475No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)