FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19534355
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA29700.079.546451
TTACGCG12500.077.7116249
TGCGCGA29000.076.9266051
TTTGCGC31200.076.4254151
ACGACCG28750.073.7793661
CGGTACG32250.073.7103351
GTGCGAC33850.072.603131
TCGCGAT21750.068.326158
TTACCCG38850.066.647981
GCGCTAG33950.066.572291
CGAACGC36350.066.402471
TAGGCGC37000.066.2243651
CCGCTAT39650.065.118781
GGCGACT38900.065.058081
GACGCGT32000.064.914711
ATAACGC36050.064.114541
GCGCGTA35550.063.9875561
GCCGGAT37550.063.501271
CCTTACG36200.063.2427181
GTACGCT28450.063.1919488
Produced by FastQC (version 0.11.5)