FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19534355
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT30350.094.34986
ACGACGC31200.093.17115
CACGACG33350.087.383284
GACGCTC42750.066.982847
TACACGA46750.062.2429582
CTACACG49200.059.636181
ACGCTCT51300.056.2428328
ACACGAC58750.049.727353
CGCTCTT67400.042.593189
TCGCCGG26607.8216544E-117.085616145
CTTCCGA106900.06.65936110-14
TCTTCCG99200.06.6354910-14
TAACCCT758100.06.26350366
AACGGGG34405.511538E-105.98551651
CTAACCC810650.05.95569325
AACCCTA819150.05.9069661
ACCCTAA835250.05.84690482
CCTAACC833500.05.8186024
CCCTAAC845700.05.7860953
TTCCGAT133750.05.63687410-14
Produced by FastQC (version 0.11.5)