FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19304895
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC30750.083.925851
CGCCGAA29350.080.705491
TTACGCG11450.080.408769
GTGCGAC33350.071.902531
TTACCCG37300.070.5601961
CGGTACG29850.070.291651
ACGACCG29650.070.2726441
TGCGCGA28700.070.051421
ATAACGC34200.068.4053341
GCGCTAG31400.067.287491
TAGGCGC35700.066.145521
CGAACGC36950.065.490711
CCTTACG36600.064.718741
CGTTGCG15250.064.650857
TCCGCGA30950.064.413548
GTACGCT27300.064.2625358
ATTAGCG39950.064.232741
GACGCGT34050.063.98311
GTACCGT42050.063.7882778
GGCGACT39200.063.596681
Produced by FastQC (version 0.11.5)