FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21114185
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC32100.078.173821
TTACGCG13550.076.507459
CGCCGAA33500.075.78041
TGCGCGA31150.073.512051
CGGTACG32250.072.138921
GCGCTAG36450.071.654411
ACGACCG31250.071.638041
GTGCGAC36050.071.0288851
CGAACGC40500.067.0179441
TCGCGAT25500.064.2504658
CCTTACG40900.064.216021
TTACCCG40250.063.798941
CGAACTA43700.063.617181
GGCGACT41550.063.563591
ATAACGC39800.063.417371
CGACACG40750.063.016131
AACCGCG37700.062.87471
CCGCTAT42550.062.757491
GACGCGT32750.062.5487981
TAGGCGC38800.062.2235071
Produced by FastQC (version 0.11.5)