FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18611467
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11050.085.944829
TGCGCGA29300.079.413851
ACGACCG27200.074.013911
GTGCGAC32850.073.274311
CGGTACG28900.072.688851
CGCCGAA31350.071.894241
TTTGCGC31950.070.544111
GACGCGT29650.067.1600651
GGCGACT38100.067.0063861
ATAACGC31600.066.478091
GCGCGTA30700.066.288621
ACGCCGA36450.066.037341
CGAACGC35950.063.9123541
TCGTACG37500.063.6024938
GTTACGC27700.063.5969128
TTACCCG36600.063.375181
TAGGCGC36850.063.3411061
CCGCTAT36850.062.3514021
TCCGCGA27350.062.025188
GCGCTAG30400.061.9040831
Produced by FastQC (version 0.11.5)