FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18611467
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC54050.0116.151335
CACGACG55000.0114.145694
CGACGCT55500.0112.594266
GACGCTC64750.096.3974157
TACACGA70550.089.731812
CTACACG71250.088.714961
ACGCTCT75800.082.440398
ACACGAC77700.081.544293
CGCTCTT86950.071.368439
CTTCCGA111450.012.57967110-14
TCTTCCG112750.011.99742310-14
TTCCGAT128400.011.03195410-14
TCCGATC126050.010.570477515-19
CCGATCT137450.09.9785715-19
CGATCTA35200.08.89678415-19
GCTCTTC211350.06.68158610-14
CGTTGAG25102.9834882E-76.1565031
CGATCTT67800.05.94479715-19
GCGTCGG27954.818521E-75.705916145
AACGGGG31007.663766E-85.6968931
Produced by FastQC (version 0.11.5)