FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16471932
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC27150.082.192831
TGCGCGA25000.079.896281
TTACGCG9550.076.669449
ACGACCG24200.074.676771
CGTTGCG12450.073.950097
GCGCTAG28350.071.4875951
CGCCGAA26500.070.680241
TTACCCG32550.068.781941
AACGCGT21650.068.308383
GTGCGAC26500.067.6431961
CCGCTAT31000.067.5006941
GCGCGTA27050.066.808791
CGAACGC30450.065.836581
GGCGACT33950.064.437011
CGGTACG27050.064.3744661
GTACCGT34900.064.185568
CCTTACG29900.063.6218221
ATAACGC31850.063.4020961
TGACGGC36250.062.16511
GACGCGT23150.061.9453931
Produced by FastQC (version 0.11.5)