FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16788475
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10000.084.8185969
TGCGCGA25350.083.643561
CGCCGAA26100.081.520151
CGGTACG24650.080.383121
ACGACCG25350.076.4329151
TTTGCGC29800.076.060271
GCGCGTA24850.073.557361
GTGCGAC28450.071.4455261
ATAACGC32550.069.185041
GGCGACT34200.066.702331
TTACCCG33800.066.626421
GCGCTAG30500.065.4448241
TAGGCGC31450.065.095341
TCGACGG29750.064.637011
CCTTACG30250.064.535451
CCGCTAT32550.064.018621
CGCGGTA33200.063.646161
GTTACCG24450.062.8582428
GCCGGAT34950.062.760531
GTTCGCG34650.062.1381658
Produced by FastQC (version 0.11.5)