FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16788475
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22200.094.374856
ACGACGC22700.093.573575
CACGACG23450.089.963324
GACGCTC32300.065.087927
TACACGA35150.061.4806142
CTACACG40550.054.270241
ACACGAC41800.051.5104643
ACGCTCT41200.051.379598
CGCTCTT52950.040.2521329
CGTTGAG23652.4556357E-107.44408751
CTTCCGA72950.07.393481310-14
TCTTCCG75000.06.824090510-14
TTCCGAT90350.06.290559310-14
CGCTGAG35252.5465852E-116.24300151
TAACCCT413150.06.01863246
CCGATCT97100.05.94284515-19
TCCGATC90100.05.88961415-19
CTAACCC430950.05.85414935
CCCTAAC447500.05.8158973
CGATGAG24357.619088E-65.723821
Produced by FastQC (version 0.11.5)