FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18125723
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10700.081.979179
TTTGCGC28650.078.66221
CGCCGAA29050.077.327191
ACGACCG25500.075.4667361
TGCGCGA26000.074.2968751
CGGTACG30350.073.532811
GACGCGT30850.073.526941
GCGCTAG31050.073.2891
GTGCGAC32200.070.898771
CCGCTAT35750.069.998711
TTACCCG33050.067.304191
GCGCGTA30600.065.280151
CGCGGTA36100.064.860861
CGAACTA41200.063.9359741
ACCGCTT41450.063.9034081
CGAACGC35050.063.8812451
ATAACGC34950.063.6453061
AACGCGT25750.063.344953
GGCGACT33400.063.3127751
TCCGCGA24650.062.93638
Produced by FastQC (version 0.11.5)