FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17024109
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10600.084.120229
TGCGCGA24550.080.18611
CGGTACG25800.077.1520541
ACGACCG25550.076.474851
TTTGCGC26300.075.685281
GTGCGAC32100.075.0048141
CGCCGAA27250.073.852371
GCGCGTA27350.068.230911
GACGCGT27850.068.057011
ATAACGC31750.065.920511
CGAACGC33300.065.4892961
TCCGCGA25500.064.8184058
TTACCCG32550.063.8506931
GCGCTAG28550.063.8251041
CCGCTAT34400.063.607891
CGCGGTA33100.062.347551
TCGTACG32850.061.5704778
GTTACGC27050.061.1042258
AACGCGT22050.060.4942673
CCTTACG32050.060.280131
Produced by FastQC (version 0.11.5)