FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17024109
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21850.092.5689246
ACGACGC22300.090.7009355
CACGACG23500.086.06944
GACGCTC33700.059.80367
TACACGA37600.053.804442
CTACACG40600.050.33581
ACGCTCT41600.049.318018
ACACGAC41650.048.562573
CGCTCTT54450.037.6791429
CGTAGAG24857.4760464E-107.07430271
TCTTCCG72550.06.694905310-14
CTTCCGA78850.06.527753410-14
CGTTGAG23851.2426244E-76.4495551
CGCAAAT24901.8249357E-65.88341471
GTTCCGA28901.5103797E-75.76955655
TAACCCT409250.05.6862876
TTCCGAT95750.05.55730710-14
CTAACCC421450.05.53888375
TCCGATC93200.05.46036115-19
CCTAACC442600.05.32334234
Produced by FastQC (version 0.11.5)