FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18582174
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG27200.079.818581
TGCGCGA27850.077.693191
CGCCGAA30400.075.985361
TTTGCGC30700.075.242831
TTACGCG11250.074.749199
CGGTACG27200.074.712341
GCGCTAG33500.073.0998461
GTGCGAC33200.070.898031
CGAACGC33550.070.8120651
GACGCGT28300.068.450381
GCGCGTA30700.067.623311
AACCGCG33750.066.493781
TAGGCGC34500.064.62451
TTACCCG35700.064.29511
ATAACGC34600.063.8039051
TCACGCG25300.063.6111878
GCCGGAT38200.063.340441
GTACCGT42750.063.2519268
GTTCGCG39000.063.1997458
TCCCGAT43000.062.7298621
Produced by FastQC (version 0.11.5)